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| Registros recuperados: 16 | |
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| TAGAWA, Michihito; TAKEUCHI, Toshihiko; FUJISAWA, Tetsuro; KONNO, Yasuhiro; YAMAMOTO, Shinji; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| A 2-year-old East Friesian sheep imported from Australia exhibited severe anemia after contagious pustular dermatitis in Hokkaido, Japan. Hemoplasma infection was confirmed in blood smears. Both Mycoplasma ovis and ‘Candidatus Mycoplasma haemovis’ were detected by PCR and sequence analyses. In the epidemiological analysis, dual pathogens were detected in 6 of 12 (50.0%) sheep imported from Australia with the infected ewe at the same time, 1 of 5 (20.0%) sheep introduced from a domestic farm in Hokkaido, and in 1 of 16 (6.3%) sheep from an epidemiologically unrelated ranch. It is the first clinical case of sheep to confirm coinfection of these pathogens in Japan. |
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Palavras-chave: 'Candidatus Mycoplasma haemovis'; Case report; Japan; Mycoplasma ovis; Sheep. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3582 |
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| SIVAKUMAR, Thillaiampalam; ASADA, Masahito; BATTSETSEG, Badgar; HUANG, Xiaohong; LAN, Dinh Thi Bich; INPANKAEW, Tawin; P. YBAÑEZ, Adrian; ALHASSAN, Andy; THEKISOE, Oriel M. M.; DE MACEDO, Alan Caine Costa; INOKUMA, Hisashi; IGARASHI, Ikuo; YOKOYAMA, Naoaki; 猪熊, 壽; 五十嵐, 郁男; 横山, 直明. |
| Babesia ovata is a tick-transmitted hemoprotozoan parasite that infects cattle. In our study, bovine blood samples (n=2,034) were collected from 10 different countries (Brazil, China, Ghana, Japan, Mongolia, the Philippines, South Africa, Sri Lanka, Thailand and Vietnam) and DNA extracted. The DNA samples were screened using an established and specific polymerase chain reaction (PCR) assay targeting the Apical membrane antigen 1 (AMA-1) gene. Parasite DNA was detected among samples collected from Japan, Mongolia and Thailand. Sequence analyses confirmed that the PCR assay detected only B. ovata AMA-1, and that amplicons from different geographical locations were conserved. Our findings highlight the importance of designing adequate strategies to control B.... |
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Palavras-chave: AMA-1; Babesia ovata; Cattle; Mongolia; Thailand. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4016 |
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| MATSUMOTO, Kotaro; TAKEUCHI, Toshihiko; YOKOYAMA, Naoaki; KATAGIRI, Yoshito; OOSHIRO, Mamoru; ZAKIMI, Satoshi; Gaowa; KAWAMORI, Fumihiko; OHASHI, Norio; INOKUMA, Hisashi. |
| We collected a total of 206 Haemaphysalis longicornis ticks by flagging in pastures in Yonaguni Island, Okinawa, Japan, in April 2008. Four of the 206 tick DNA samples tested were positive in a polymerase chain reaction (PCR) screening for the 16SrRNA gene of Anaplasmataceae. Partial sequences of 4 PCR products were identical to each other. Longer sequences of the 16SrRNA gene were successfully determined in 2 of the 4 tick samples, and the obtained 1,392 bp and 1,300 bp sequences revealed high similarity to the 16SrRNA gene sequences of the validated Ehrlichia species, including Ehrlichia ewingii, E. chaffeensis, and E. canis (98.3–98.6%). We also sequenced 1,304 bp of the groEL gene from the 2 tick samples, and found that these had the highest similarity... |
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Palavras-chave: 16SrRNA; Ehrlichia; GroEL; Haemaphysalis longicornis. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3581 |
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| YBAÑEZ, Adrian Patalinghug; MATSUMOTO, Kotaro; KISHIMOTO, Toshio; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| Anaplasma phagocytophilum causes human granulocytic anaplasmosis (HGA) and tick-borne fever in ruminants. A closely related and potentially novel Anaplasma sp. in Japan was recently characterized. The aims of the study were to provide molecular evidence for the presence of these 2 species in Japan, and to develop a reliable PCR method based on the nucleotide differences within the citrate synthase (gltA) gene. DNA samples from 182 ixodid ticks (134 Ixodes persulcatus, 35 Haemaphysalis douglasii and 13 I. ovatus) collected from 2 sites in Hokkaido, Japan, were screened for A. phagocytophilum and its closely related Anaplasma sp. (herein designated as Anaplasma sp. Japan) using 16S rRNA PCR, revealing a combined prevalence rate of 27.5% (50 samples). The... |
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Palavras-chave: 16S rRNA; Anaplasma phagocytophilum; Anaplasma sp.; GltA; GroEL. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3839 |
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| WEERASOORIYA, Gayani; SIVAKUMAR, Thillaiampalam; LAN, Dinh Thi Bich; LONG, Phung Thang; TAKEMAE, Hitoshi; IGARASHI, Ikuo; INOUE, Noboru; YOKOYAMA, Naoaki. |
| A PCR-based survey of hemoprotozoa parasites detected Babesia bigemina, Theileria orientalis and Trypanosoma theileri among cattle and water buffalo in Vietnam, and a new Babesia sp. closely related to Babesia ovata was detected in cattle only. In addition, Theileria annulata and Trypanosoma evansi were not detected in both cattle and water buffalo. Phylogenetic analysis detected T. orientalis MPSP genotypes 3, 5, 7 and N3 in cattle and 5, 7, N1 and N2 in water buffalo. Additionally, water buffalo-derived T. theileri CATL sequences clustered together with a previously reported cattle-derived sequence from Vietnam. This is the first report of a new Babesia sp. in cattle, and T. orientalis MPSP genotype 7 and T. theileri in water buffalo in Vietnam. |
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Palavras-chave: Cattle; Epidemiology; Hemoprotozoa; Vietnam; Water buffalo. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4344 |
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| BATICADOS, Waren N.; WITOLA, William H.; INOUE, Noboru; KIM, Jung-Yeon; KUBOKI, Noritaka; XUAN, Xuenan; YOKOYAMA, Naoaki; SUGIMOTO, Chihiro. |
| During the attempt to seek T. congolense species-specific diagnostic antigens, we discovered one cDNA clone (P74) encoding 74 kDa putative abc1 protein (p74) from T. congolense PCF cDNA library. It has been suggested that members of the abc1 family are novel chaperonins and essential for both the mitochondrial electron transfer in the bc 1 complex and the coenzyme Q biosynthesis. Although abc1 protein in yeast has a nuclear or mitochondrial subcellular location, neither nuclear localization signal nor mitochondrial targeting signal was found within p74. Northern blot analysis revealed that the transcription level of P74 mRNA in bloodstream form (BSF) cells were 4 times higher than that in procyclic form cells. Western blot analysis also indicated that p74... |
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Palavras-chave: Trypanosoma congolense; Abc1 family protein; Coenzyme Q. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4165 |
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| YBAÑEZ, Adrian Patalinghug; SIVAKUMAR, Thillaiampalam; YBAÑEZ, Rochelle Haidee Daclan; RATILLA, Jowarren Catingan; PEREZ, Zandro Obligado; GABOTERO, Shirleny Reyes; HAKIMI, Hassan; KAWAZU, Shin-ichiro; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 河津, 信一郎; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| Anaplasma marginale has been detected in the Philippines only by peripheral blood smear examination and serological methods. This study generally aimed to molecularly detect and characterize A. marginale in cattle and ticks in Cebu, Philippines. A total of 12 bovine blood samples and 60 Rhipicephalus (Boophilus) microplus ticks were collected on the Cebu Island in 2011. 16S rRNA-based screening-PCR and DNA sequencing revealed 8 cattle (66.7%) and 8 ticks (13.3%) to be positive for A. marginale, and 1 tick (1.7%) to be positive for A. centrale. Selected positive DNA samples were further characterized based on 16S rRNA (longer sequence), Msp5, Msp1α,gltA and groEL genes for phylogenetic analyses. Sequence identities of partial DNA fragments of A. marginale... |
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Palavras-chave: Anaplasma marginale; Cattle; PCR; Philippines; Rhipicephalus (Boophilus) microplus. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3866 |
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| YOKOYAMA, Naoaki; UENO, Akio; MIZUNO, Daisuke; KUBOKI, Noritaka; KHUKHUU, Altangerel; IGARASHI, Ikuo; MIYAHARA, Tokuji; SHIRAISHI, Takashi; KUDO, Ryuta; OSHIRO, Mamoru; ZAKIMI, Satoshi; SUGIMOTO, Chihiro; MATSUMOTO, Kotaro; INOKUMA, Hisashi; 横山, 直明; 五十嵐, 郁男; 松本, 高太郎; 猪熊, 壽. |
| Theileria orientalis is a benign protozoan species that is widely distributed in Japan, yet sometimes causes serious economic losses in the livestock industry. In this study, we conducted a molecular survey based on genes encoding the major piroplasm surface protein (MPSP) and p23 for T. orientalis detected in cattle grazing in southern areas of Japan, consisting of 2 farms in Kumamoto prefecture (Aso and Kuma districts) and 3 farms in Okinawa prefecture (Ishigaki, Iriomote, and Yonaguni Islands). High prevalence rates of T. orientalis infection were shown in all the cattle populations using the diagnostic MPSP- and p23-PCR assays. Phylogenetic analyses revealed 4 MPSP genotypes and 3 p23 genotypes. Furthermore, MPSP genotype-specific PCR methods were... |
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Palavras-chave: Cattle; Genotype-specific PCR; Japan; MPSP gene; Theileria orientalis. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3584 |
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| TAKABATAKE, Noriyuki; OKAMURA, Masashi; YOKOYAMA, Naoaki; OKUBO, Kazuhiro; IKEHARA, Yuzuru; IGARASHI, Ikuo; 横山, 直明; 五十嵐, 郁男. |
| Host sialic acid (SA) has recently been suggested to play an important role in erythrocyte (RBC) infection by Babesia spp. The present study attempted to further determine the specific type of SAs important in the RBC invasion. Bovine RBC was found to bear abundant α2-3-linked SA residues but not α2-6-linked SA in nature, confirmed by flow cytometric analysis of the neuraminidase (Nm)-treated RBCs. Lectin-blot analyses revealed the removal of α2-3-linked SAs from the 97-, 33-, and 31-kDa bands by the Nm treatment. Addition of the Nm-treated RBCs into an in vitro culture of B. bovis resulted in a decreased population of the parasitized RBCs. The thin smear samples from the cultures were then observed under a confocal laser scanning microscope after staining... |
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Palavras-chave: Babesia bovis; Erythrocyte; Invasion; Sialic acid. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4166 |
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| KHUKHUU, Altangerel; LAN, Dinh Thi Bich; LONG, Phung Thang; UENO, Akio; LI, Yan; LUO, Yuzi; MACEDO, Alan Caine Costa de; MATSUMOTO, Kotaro; INOKUMA, Hisashi; KAWAZU, Shin-Ichiro; IGARASHI, Ikuo; XUAN, Xuenan; YOKOYAMA, Naoaki; 松本, 高太郎; 猪熊, 壽; 河津, 信一郎; 五十嵐, 郁男; 玄, 学南; 横山, 直明. |
| Theileria orientalis is a benign bovine protozoan parasite that occasionally causes serious economic loss in the livestock industry. We report the findings of a molecular epidemiological survey of T. orientalis in 94 Vietnamese yellow cattle, 43 water buffaloes, 21 sheep, 21 goats and 85 blood-sucking ticks of cattle in the Thua Thien Hue province of Vietnam. The major piroplasm surface protein (MPSP) gene of T. orientalis was detected using polymerase chain reaction from 13 cattle (13.8%), 11 water buffaloes (25.6%), 1 sheep (4.8%) and 9 ticks (10.6%). Phylogenetic analysis using MPSP gene sequences showed the presence of seven genotypes, four previously categorized genotypes (Types 1, 3, 5 and 7) and three new genotypes (Types N-1, N-2 and N-3). |
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Palavras-chave: Cattle; Genotype; MPSP; Theileria orientalis; Vietnam. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3585 |
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| SIVAKUMAR, Thillaiampalam; LAN, Dinh Thi Bich; LONG, Phung Thang; YOSHINARI, Takeshi; TATTIYAPONG, Muncharee; GUSWANTO, Azirwan; OKUBO, Kazuhiro; IGARASHI, Ikuo; INOUE, Noboru; XUAN, Xuenan; YOKOYAMA, Naoaki; 井上, 昇; 玄, 学南; 横山, 直明. |
| Hemoprotozoan infections often cause serious production losses in livestock. In the present study, we conducted a PCR-based survey of Babesia bovis, Babesia bigemina, Theileria annulata, Theileria orientalis, Trypanosoma evansi and Trypanosoma theileri, using 423 DNA samples extracted from blood samples of cattle (n=202), water buffaloes (n=43), sheep (n=51) and goats (n=127) bred in the Hue and Hanoi provinces of Vietnam. With the exception of T. annulata and T. evansi, all other parasite species (B. bovis, B. bigemina, T. orientalis and T. theileri) were detected in the cattle populations with B. bovis being the most common among them. Additionally, four water buffaloes and a single goat were infected with B. bovis and B. bigemina, respectively. The Hue... |
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Palavras-chave: Epidemiology; Hemoprotozoa; Livestock; PCR; Vietnam. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4015 |
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| TAGAWA, Michihito; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| Hemotropic mycoplasmas (hemoplasmas) are cell-wall deficient, epierythrocytic bacteria that cause infectious anemia in several mammalian species. The prevalence of hemoplasma species was examined by screening and species-specific PCR using blood samples collected from 51 sika deer in Hokkaido, Japan. Molecular analyses were performed for the 16S rRNA, 23S rRNA and RNase P RNA (rnpB) gene sequences. A total of 23/51 (45%) deer DNA samples were positive for hemoplasmas in the screening PCR. Using species-specific PCR, 12 and 17 samples were positive for ‘Candidatus Mycoplasma haemocervae’ and ‘Candidatus M. erythrocervae’, respectively. Sequencing and phylogenetic trees of those three genes indicate that the ‘Candidatus M. haemocervae’ and ‘Candidatus M.... |
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Palavras-chave: Cervus nippon yesoensis; Hemoplasma; Japan; Phylogeny. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4012 |
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| TAGAWA, Michihito; YBANEZ, Adrian P.; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| The prevalence and risk factors of 2 bovine hemoplasma species—Mycoplasma wenyonii (Mw) and “Candidatus Mycoplasma haemobos” (CMh)—were examined by direct PCR using whole blood samples collected from 343 cattle in Eastern Hokkaido, Japan. The sensitivity of the direct PCR assay was 10-fold higher than the standard PCR assay, detecting 5 copies of bovine hemoplasma DNA per reaction. An epidemiological survey of sampling location, living conditions, age and bovine leukemia virus antibody was performed to assess infection risk factors. The prevalence rates were 38.5% for Mw and 39.1% for CMh, respectively, with an overall prevalence rate of 64.7% for all bovine hemoplasma infections. Significant differences were found in sampling location, living conditions... |
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Palavras-chave: Bovine hemoplasma; Direct PCR; Japan; Risk factor. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3838 |
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| YBAÑEZ, Adrian Patalinghug; SIVAKUMAR, Thillaiampalam; BATTSETSEG, Badgar; BATTUR, Banzragch; ALTANGEREL, Khukhuu; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| Anaplasma marginale is an etiologic agent of bovine anaplasmosis. This study aimed to molecularly detect and characterize A. marginale that is prevalent in Mongolian cattle populations. A highly specific and sensitive nested PCR (nPCR) method based on the Msp5 gene was developed to detect A. marginale (Msp5 nPCR). The method detected A. marginale from the positive DNA samples obtained from different countries, while no amplicons were observed from DNA samples of several other bovine blood pathogens tested. The detection limit of Msp5 nPCR was determined to be 2 copies/μl. The method was tested against field blood DNA samples prepared from 300 Mongolian cattle in 2010. Results indicated a prevalence rate of 8.7% (26 of 300). On the other hand, partial DNA... |
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Palavras-chave: Anaplasma marginale; Cattle; Mongolia; Msp5; PCR. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3867 |
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| NAGANO, Daisuke; SIVAKUMAR, Thillaiampalam; DE DE MACEDO, Alane Caine Costa; INPANKAEW, Tawin; ALHASSAN, Andy; IGARASHI, Ikuo; YOKOYAMA, Naoaki; 五十嵐, 郁男; 横山, 直明. |
| In the present study, we screened blood DNA samples obtained from cattle bred in Brazil (n=164) and Ghana (n=80) for Babesia bovis using a diagnostic PCR assay and found prevalences of 14.6% and 46.3%, respectively. Subsequently, the genetic diversity of B. bovis in Thailand, Brazil and Ghana was analyzed, based on the DNA sequence of merozoite surface antigen-1 (MSA-1). In Thailand, MSA-1 sequences were relatively conserved and found in a single clade of the phylogram, while Brazilian MSA-1 sequences showed high genetic diversity and were dispersed across three different clades. In contrast, the sequences from Ghanaian samples were detected in two different clades, one of which contained only a single Ghanaian sequence. The identities among the MSA-1... |
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Palavras-chave: Babesia bovis; Brazil; Ghana; MSA-1; Thailand. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4014 |
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| 猪熊, 壽; 横山, 直明; INOKUMA, Hisashi; YOKOYAMA, Naoaki. |
| 北海道東部(道東)の放牧地における牛の小型ピロプラズマ感染りスク低減要因について調査した。道東ではシュルツェマダニ，ヤマトマダニ，ダグラスチマダニが採取され， PCRにより小型ピロプラズマ遺伝子断片が検出され，これらのマダニがベクターとなりうる可能性が考えられた。エゾシカからは多数のマダニが回収され，小型ピロプラズマのベクターを牛の放牧地内へ持ち込むという意味で小型ピロプラズマ感染リスクに関わる動物と考えられた。また，放牧管理体制との関連では，野生動物侵入防止柵の設置，あるいは牧柵周辺の下草刈りが小型ピロプラズマ感染リスク低減に有効である可能性が考えられた。 Factors to decrease risk for Theileria orientalis infection of cattle in pastureland in Eastern Hokkaido were investigated. Ixodes persulcatus，Ixodes ovatus and Haemaphysalis douglusii were collected from the vegetation of the pastureland，and DNA fragments of T. orientalis were detected from these ticks by using PCR. The results suggested that these tick species may be vectors for T. orientalis in this area. Many ticks have been recovered from wild sika deer，and it was thought... |
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Palavras-chave: 道東; リスク低減要因; Theileria orientalis; Eastern Hokkaido; Factors to decrease risk. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2790 |
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| Registros recuperados: 16 | |
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